How you can calibrate your SPR measurement
Calibration protocol for P4SPR
P4SPR™ Series
Core Technology
Basic thin film SPR found in most benchtop devices capable of detection in complex media such as serum, plasma, cell lysates, or wastewater.
Flexible Design
Adaptable from injection to sensor. Comes with two injection models with multiple options to meet your research needs
Rapid Data Processing
Intuitive software providing key data for biosensing and protein interaction characterization in real time.
Dual Inlet Model
Best for assay development
-
Triplicate sample channel measurement with reference channel
-
Connect to Affinité’s injection loop side module for kinetics analysis
Dual Inlet Model
Quad Inlet Model
Best for intermediate screening
-
Simultaneous comparison of four different samples at once.
-
Fewer chip consumable usage.
Quad Inlet Model
-
Thin film-based SPR
-
Dimensions: 175 x 155 x 55 mm
-
Weight: < 1.3 kg.
-
USB powered
-
Microfluidic cell min. volume: 50 uL
-
Sensitivity: 2750 nm/RIU
-
Resolution : 1 micro RIU
-
Dynamic range: 1.33 to 1.39 refractive index unit
-
Coefficient of variation on signal: < 0.6%
-
Polychromatic light source
-
P4SPR Control™ graphical user interface
-
ezControl graphic user interface
-
Output data compatible with TraceDrawer™
With P4SPR, we were able to establish optimal immobilization conditions of SARS-CoV-2 spike and nucleocapsid within 2-3 SPR runs. Having the device at our bench also really helped comparing our SPR results against ELISA.
- Abdelhadi, M.Sc., Research Assistant, Université de Montréal