Affinité Expertise

Enhanced sensitivity with microhole arrays ​

The analytical technique surface plasmon resonance (SPR) is mainly defined by two classes of plasmonic materials: nanoparticles (mostly nanospheres) and thin films. Both classes exhibit nanometric physical features and present distinct optical characteristics fitting different applications. For instance, nanoparticles are the most sensitive materials but they cannot be deployed in complex media such biofluids (e.g., blood, serum, urine) for SPR measurements as the media will generate optical interferences.

Although less sensitive, thin films address all the complex media issue in addition to be easier to manufacture for large scale deployment in industry applications. Effort to combine the strength of both plasmonic materials into a single one have been driven by researchers. Most successful efforts started from the thin film end of the spectrum and moved towards integrating nanoparticle features to thin films.

Lower non-specific adsorption with Afficoat

The Afficoat forms a protective self-assembled monolayer on metallic materials that significantly improves molecular recognition by minimizing non-specific adsorption. The proprietary heteropeptide sequence of Afficoat has proven to be better than other broadly used anti-fouling chemistry such as Dextran, PEG-COOH and alkanethiols. 

SPR Sensors & Microfluidics

Glass chips

Bare glass SPR sensor chip for customization through PVD or sputtering metalization.

Au sensors

Pack of 5 bare Au coated chips. Ideal for aptamer testing.

Au sensors + 16-MHA

Pack of 5 16-MHA coated Au SPR chips - 16-MHA is a SAM that reduces nonspecific binding. Ideal for immunoassays

Au sensors + Afficoat

Pack of 5 Afficoated Au SPR chips - Afficoat is a peptide surface chemistry that reduces non-specific binding. Ideal for immunoassays.

Au sensors + Ni-NTA:

Pack of 5 Ni-NTA coated Au SPR chips - Ni-NTA is often used in affinity columns to purify His-tagged proteins. Ideal for immunoassays and His-tagged proteins.

Au sensors + Streptavidin

Pack of 5 streptavidin coated Au SPR chips - Streptavidin forms a strong bond with biotin. Ideal for immunoassays and biotinylated proteins.

Calibration chip set:

Test the sensitivity of your batch of chips or your overall system performances with the calibration kit. This kit comes with three Au sensors and all the solutions to perform at least 10 tests.

DNA-Protein chip set:

Learn how to perform a DNA-Protein test and measure a dissociation constant on P4SPR using the LacI/LacO interaction as model. It comes with five Ni-NTA coated chips and all the solutions to run three tests.

DNA-DNA chip set

Learn how to perform a DNA-DNA test on P4SPR. This kit comes with five bare Au chips and all the solutions to run three tests.

Protein-protein chip set

Learn how to perform a protein-protein test to obtain a dissociation constant on P4SPR using the IgG/anti-IgG interaction as a model. This kit comes with five bare Au chips and all the solutions to run three tests.

Microfluidic cell

A pack of three microfluidic cells with 3 + 1 channels: two inlets feeding an “S” shaped channel for sample and a linear one for reference.

Blunt end needle

10 blunt end needles for direct injection size: 18G, 1/4 inch