Research & Development

Quantitative measurement of a drug or biomarker in biological fluids, primarily blood, plasma, serum, urine or tissue.

Assessment of the quality of the environmental parameters in order to control the risk of pollution and potential harm to humans.

 

Discovering new plasmonic and chemical materials. Exploiting their optical properties for biosensing applications.

 

Cross-Validation of ELISA and a Portable Surface Plasmon Resonance Instrument for IgG Antibodies Serology with SARS-CoV-2 Positive Individuals
ChemRxiv
2021
Clinical Analysis
An orally available PD-1/PD-L1 blocking peptide OPBP-1-loaded trimethyl chitosan hydrogel for cancer immunotherapy
Journal of Controlled Release
2021
Clinical Analysis
Heparin Inhibits Cellular Invasion by SARS-CoV-2: Structural Dependence of the Interaction of the Spike S1 Receptor-Binding Domain with Heparin
Biosensor and Bioelectronics
2020
Biosensing
Portable and field-deployed surface plasmon resonance and plasmonic sensors
Analyst
2020
Biosensing
A rapid and quantitative serum test for SARS-CoV-2 antibodies with portable surface plasmon resonance sensing
ChemRxiv
2020
Clinical Analysis
SARS-CoV-2 Spike S1 Receptor Binding Domain undergoes Conformational Change upon Interaction with Low Molecular Weight Heparins
BioRxiv
2020
Durg Discovery
Three-dimensional biosensor surface based on novel thorns-like polyelectrolytes
Biosensors and Bioelectronics
2020
Biosensing
The 2019 coronavirus (SARS-CoV-2) surface protein (Spike) S1 Receptor Binding Domain undergoes conformational change upon heparin binding
BioRxiv
2020
Biosensing
Fundamentals of real-time surface plasmon resonance-electrogenerated chemiluminescence
Angewandte Chemie International Edition
2019
Material
Polymer-Templated Gold Nanoparticles on Optical Fibers for Enhanced-Sensitivity Localized Surface Plasmon Resonance Biosensors
ACS Sensors
2019
Material
Miniaturized Surface Plasmon Resonance based Sensor Systems-Opportunities and Challenges
Optics, Photonics and Laser Technology 2018
2019
Instrumentation
Optical biosensors and Applications to Drug Discovery for Cancer Cases
Biosensors and Nanotechnology: Applications in Health Care Diagnostics
2018
Biosensing
Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance
Antibody engineering-Methods and Protocols: 3rd Edition
2018
Biosensing
Surface Plasmon Resonance–Based Immunoassays: Approaches, Performance, and Applications
Handbook of Immunoassay technologies: Approaches, Performance, and Applications
2018
Immunoassay
Surface Plasmon Resonance: Material and Interface Design for Universal Accessibility
Analytical Chemistry
2018
Biosensing
Hybridization conditions of oligonucleotidecapped gold nanoparticles for SPR sensing of microRNA
Biosensors and Bioelectronics
2018
Drug Discovery
Tracking silent hypersensitivity reactions to asparaginase during leukemia therapy using single-chip indirect plasmonic and fluorescence immunosensing
ACS Sensor
2017
Biosensing
Development of E. coli asparaginase II for immunosensing: A trade-off between receptor density and sensing efficiency
ACS Omega
2017
Biosensing
Surface Plasmon Resonance Clinical Biosensors for Medical Diagnostics
ACS Sensors
2017
Biosensing
Epstein-Barr virus induced gene 3 AEBI3B can mediate ILZ6 trans-signaling
The Journal of Biological Chemistry
2017
Drug Discovery
Development of E. coli asparaginase II for immunosensing: A trade-off between receptor density and sensing efficiency
ACS Omega
2017
Biosensing
Epstein-Barr virus induced gene 3 AEBI3B can mediate ILZ6 trans-signaling
The Journal of Biological Chemistry
2017
Drug Discovery
Surface Plasmon Resonance Instruments
Handbook of Surface Plasmon Resonance: 2nd Edition
2017
Instrumentation
Ultra-low fouling methylimidazolium modified surfaces for the detection of HER2 in breast cancer cell lysate
Analyst
2017
Biosensing
Portable instrumentation & point-of-care technologies
Analytical Methods
2016
Biosensing
Response monitoring of acute lymphoblastic leukemia patients undergoing LAsparaginase therapy: successes and challenges associated with clinical sample analysis in plasmonic sensing
ACS Sensors
2016
Biosensing
Biosensors and nanobiosensors for therapeutic drug and response monitoring
Analyst
2016
Biosensing
A CD36 ectodomain mediates insect pheromone detection via a putative tunnelling mechanism
Nature Communications
2016
Drug Discovery
Surface plasmon resonance based miniaturized biosensors for medical applications
Nanobiosensors for Personalized and Onsite Biomedical Diagnosis
2016
Biosensing
Will Nanobiosensors Change Therapeutic Drug Monitoring? The Case of Methotrexate
Nanomedicine-UK
2015
Biosensing
Influence of the Particle Shape and Density of Self- Assembled Gold Nanoparticle Sensors on LSPR and SERS
J. Phys. Chem. C
2015
Material
Single chip SPR and fluorescent ELISA assay of prostate specific antigen
Lab on Chip
2015
System Integration
Microdialysis SPR: Diffusion-Gated Sensing in Blood
Chemical Science
2015
System Integration
Label-Free Technologies for Monitoring Drug Interactions
Drug Target Review
2015
Biosensing
Plasmonic sensors for the competitive detection of testosterone
Analyst
2015
Biosensing
Plasmonic Sensors for Analysis of Proteins and an Oncologic Drug in Human Serum
Nanobiosensors and Nanobioanalyses
2015
Biosensing
Miniature Multi- Channel SPR Instrument for Methotrexate Monitoring in Clinical Samples
Biosensor and Bioelectronics
2015
Biosensing
Non-specific Adsorption of Crude Cell Lysate on Surface Plasmon Resonance Sensors
Langmuir
2013
Sensing
Nanostructured Substrates for Portable and Miniature SPR Biosensors
Analytical Bioanalytical Chemistry
2012
Material
Modified peptide monolayer binding His-tagged biomolecules for small ligand screening with SPR biosensors
Analyst
2011
Biosensing
Propagating Surface Plasmon Resonance on Microhole Arrays
Analytical Chemistry
2010
Sensing
Peptide Self-Assembled Monolayers for Label-Free and Unamplified Surface Plasmon Resonance Biosensing in Crude Cell Lysate
Analytical Chemistry
2009
Biosensing
Bioanalytical Protein detection

Approximately one in five women diagnosed with breast cancer worldwide will have HER2-positive breast cancer, a particularly aggressive form of the disease. Although four diagnostic tests exist, research has shown that some HER2 status test results may still be wrong. 

A diagnostic test using SPR may help address some of the current issues. The potential biosensing application of a new ultra-low fouling ionic liquid is demonstrated using the P4SPR for the analysis of HER2 in breast cancer cell lysates. 

Bioanalytical Small peptide detection

CD36 is a scavenger receptor important in age-related macular degeneration and other diseases. CD36 transmembrane proteins have diverse roles in lipid uptake, cell adhesion and pathogen sensing.

 

Despite numerous in vitro studies, how they act in native cellular contexts is poorly understood. In an investigation with a Drosophila CD36 homologue, SNMP1, the P4SPR was employed to assess small-molecule interactions with a CD36 sensor chip.

Bioanalytical DNA detection

Cytokine release syndrome is a form of systemic inflammatory response syndrome that arises as a complication of some diseases or infections, and is also an adverse effect of some monoclonal antibody drugs and other treatments. Severe cases have been called “cytokine storms” and can be fatal.

 

Understanding the mechanism of inflammation is important. Here, the P4SPR was deployed to investigate the binding of a gene (EBI3) to IL-6, a pro-inflammatory cytokine. This unexpected observation could lead to a new therapeutic biologic for autoimmune diseases. 

Bioanalytical Protein detection

E. coli L-asparaginase II (EcAII) is a critical component of chemotherapy for childhood acute lymphoblastic leukemia (ALL). This therapeutic, listed as essential medicine by the WHO since 1995, may be compromised by allergic reactions, overt or silent. The main concern relative to the silent hypersensitivity that occurs in 5-46% of patients is the development of neutralizing antibodies that result in silent inactivation of the treatment.

In order to monitor the efficacy of the treatment with EcAII, a test to detect the anti-EcAII antibodies (neutralizing antibodies) using the P4SPR. SPR bioassays were performed in undiluted serum from children undergoing therapy for ALL.

Bioanalytical Cancer therapeutic

Methotrexate (MTX) is one of the most widely studied and effective therapeutics agents available to treat many solid tumors, hematologic malignancies, and autoimmune diseases such as rheumatoid arthritis; however, the poor pharmacokinetic and narrow safety margin of the drug limits the therapeutic outcomes of conventional drug delivery systems.

MTX concentrations in a patient’s serum undergoing chemotherapy treatments can be determined by surface plasmon resonance (SPR) sensing using folic acid-functionalized gold nanoparticles (FA-AuNP) in competition with MTX for the bioreceptor, human dihydrofolate reductase (hDHFR) immobilized on the SPR sensor chip.

Image by Jens Johnsson

Rapid detention explosives (RDX) have been used extensively in the manufacture of munitions and accounts for a large part of the explosives contamination at active and former military installations in various parts of the world. Most RDX are not significantly retained by most soil and biodegrade very slowly.

 

As a result, it can easily migrate to groundwater. They are classified as possibly carcinogenic and can damage the nervous system if inhaled or ingested.

 

See how the P4SPR was deployed on the field to test RDX in water at levels near the EPA limit of 2 ppb.